Cell-Based Sensors for the Detection of EGF and EGF-Stimulated Ca2+ Signaling
Cell-Based Sensors for the Detection of EGF and EGF-Stimulated Ca2+ Signaling
Blog Article
Ott-Antonsen ansatz truncation of a circular cumulant series
.Epidermal growth factor (EGF)-mediated activation of EGF receptors (EGFRs) has become an important target in drug development due to the implication of EGFR-mediated cellular signaling in cancer development.While various in vitro approaches are developed for monitoring EGF-EGFR interactions, they have several limitations.Herein, we describe a live cell-based sensor system that can be used to monitor the interaction of EGF and EGFR as well as the subsequent signaling events.
The design of the EGF-detecting sensor cells is based on the split-intein-mediated conditional protein trans-cleavage reaction (CPC).CPC is triggered by the presence of the target (EGF) to activate a signal peptide that translocates the fluorescent cargo to the target cellular location (mitochondria).The developed sensor cell demonstrated excellent sensitivity with a fast response time.
It was also successfully used to detect an agonist and antagonist of EGFR (transforming growth factor-α and Cetuximab, respectively), demonstrating excellent specificity and capability of screening the analytes based on their function.
Revisiting the Hermeneutic Project of the Fragment in its Exigency for a Digital Paradigm of Publication
.The usage of sensor cells was then expanded from merely detecting the presence of target to monitoring the target-mediated signaling cascade, by exploiting previously developed Ca2+-detecting sensor cells.These sensor cells provide a useful platform for monitoring EGF-EGFR interaction, for screening EGFR effectors, and for studying downstream cellular signaling cascades.